Abstract
Two dimensional thin layer Chromatographic techniques have been used in the study of the carotenoid pigments in the seeds of Bixa orellana L. In addition to the two major carotenoids bixin and norbixin,β-carotene, cryptoxanthin, lutein, zeaxanthin and methyl bixin have been tentatively identified by co-chromatography. There were other unidentified carotenoids in trace amounts. The probable biosynthetic significance of these carotenoids in relation to bixin is discussed.
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