Abstract
The biosynthesis pathway of 3-isopropyl-2-methoxypyrazine (1) by Pseudomonas taetrolens was studied. Cultivation of the bacterium in a broth containing l-leucine as a unique source of carbon and nitrogen allowed an improvement of the yield of 1 which was up to 10 mg/litre. 13C- l-valine was introduced into the medium. Involvement of the amino-acid in the 1 biosynthesis could be proved by following the incorporation of the labelling by both MS and 1H and 13C-NMR. Attempts to introduce 13C-glycine (which was supposed to be the second precursor) into the 1 molecule were unsuccessful.
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