STUDY OF THE ANTI-HYPERLIPIDEMIC EFFECT OF THE COMBINED ADMINISTRATION OF THREE NATURAL EXTRACTS IN A POLOXAMER-407 HYPERLIPIDEMIC MODEL AND THEIR LC-ESI-MS/MS2 AND HPLC PROFILING

Abstract

Objective: Dyslipidaemia is considered a high-risk factor for inducing atherosclerosis and cardiovascular diseases (CVDs). This study aims to investigate the anti-hyperlipidemic effect of the co-administration of the ethanol extracts of both ginger (root and rhizome) and leek (leaves and bulbs) in addition to the aqueous extract of gum arabic. 
 Methods: Rats were divided into eight groups: Hyperlipidaemia was induced in rats by a single intraperitoneal injection of Poloxamer 407 (P-407) [1 g/kg], negative control [saline injected], hyperlipidemic control [P-407 injected], positive control [Atorvastatin 70 mg/kg], groups four, five and six received ginger extract (400 mg/kg), leek extract (500 mg/kg) and gum arabic aqueous extract (7.5 g/kg) respectively and groups seven and eight received a co-administration of ginger, leek and gum arabic extracts at doses A and B respectively. Lipid profile was monitored. The profiling of all the tested extracts was performed by LC-ESI/MS and HPLC.
 Results: A significant anti-hyperlipidemic activity (P<0.05) was seen for group eight among all the tested groups producing ≈54%, 72%, 50% and 72% decrease in the measured parameters total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) respectively. An overall of 56 and 45 compounds were tentatively identified in the ethanol extracts of ginger and leek, respectively. Galactose and arabinose sugars were found to be the major saccharides in gum arabic and glucuronic acid was the major polyuronide part.
 Conclusion: the co-administration of a group of natural extracts in the given concentration proved to be more effective than the use of synthetic drugs or the use of a single component.