Study of the acute toxicity and cumulative properties of the combined drug based on imidacloprid, pyriproxyfen and moxidectin

Abstract

The purpose of the research is the study of acute oral and dermal toxicity, and cumulative properties of the combined drug based on imidacloprid, pyriproxyfen and moxidectin on mice and rats.Materials and methods. The studies were conducted as provided by the Guidelines of the State PharmacologicalCommittee, in the VNIIP – FSC VIEV vivarium in 2021. We studied the acute oral and dermal toxicity, as well as cumulative properties of the combined drug in the form of a solution for external use that contains imidacloprid, pyriproxyfen and moxidectin as active substances. Outbred male mice and male rats were used in studying toxicological characteristics of the drug. General methods were used in studying the acute oral toxicity in the mice and rats, acute dermal toxicity in the rats and cumulative properties of the prototype product in the mice.Results and discussion. The LD 50 of the prototype product was 800 mg/kg of the animal weight when administered orally to the mice, and 2520±916.7 mg/kg, to the rats. Subject to the established median lethal doses, the drug was classified as the 3rd hazard class according to the general hygienic classification (GOST 12.1.007-76). When studying the acute dermal toxicity in the rats, the LD50 of the drug exceeded the maximum possible dose of 10,000 mg/kg. According to the general hygienic classification (GOST 12.1.007-76), the drug was classified as the 4th hazard class. The accumulation factor was 8.25, in which case the drug can be classified as the group of substances with weak cumulative activity.