Study of synergistic Inhibitory effectiveness of mixed of Sweet bell pepper extract and virgin olive oil in α -amylase and α-glucosidase activity in serum of male rats infected with experimental diabetes

Abstract

The present research designed for evaluate the inhibitory activities of red bell pepper alone ,red bell pepper and virgin olive oil together on alpha-amylase and alpha-glucosidase activities of adult male rats (Rattus norvegicus) infected with experimental Diabetes induced by alloxan, in present study used (40) male rats weight (250-300) gram, their age (14-16) weeks the experimental animals had been bought from faculty of veterinary/ university of mousl and had been performed in the animal house at Dept. of Biology of science college at Tikrit Univ. from October to December, The present study intend to screen novel alpha amylase and alpha glucosidase inhibitors from natural sources to management of diabetes mellitus type 2.
 1- The results had been shown significant decrease at the level of (p<0.01) in serum glucose concentration in diabetics rats given mixed of red bell pepper and virgin olive oil in dose –dependent manner compare with diabetic group alone.
 2- high significant increase (P<0.01) in insulin concentration in diabetics male rats given mixed red bell pepper and olive oil compared with diabetic group alone . 
 3- The present result had been shown high inhibitory percentage of mixed extract of red bell pepper and virgin olive oil together against alpha-amylase and alpha-glucosidase in diabetic rats more than red bell pepper alone in dose-dependent manner (2 ml, 4 ml, 6 ml, 8 ml/ kg. of body weight).
 Conclusion: the mixture consist of red bell pepper extract and virgin olive oil show positive effect on inhibitory of amylase activity and alpha-glucosidase but effect on alpha- amylase more than on alpha glucosidase activity in male diabetic rats this may be considered this mixture as one of good management of hyperglycemia