Abstract
The cause of the majority of surgically excised pulmonary granulomas has not been determined, even with extensive bacteriological studies. However, through application of Gomori's methenamine-silver nitrate stain (GMS) to 138 such surgically excised lesions over a two-and-a-half-year period, it was possible to identify structures resembling Histoplasma capsulatum or Coccidioides immitis in 55.9%. Despite extensive efforts, it was not possible to obtain fungus growths from these structures, which are thought to be nonviable fungi that have lost their ordinary stainability. With the GMS stain, fungi can be found in tissue sections more rapidly and under lower magnification than by other staining methods.
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