Abstract
A modified procedure for the use of the cysteine – sulfuric acid reagent for the determination of monosaccharide has been devised. The procedure utilizes a 9-ml volume of H2O:H2SO4 at a ratio of 1:7 which results in a rapid nonspecific method for the determination of pentoses, hexoses, and hexuronic acids in simple solutions. α-Substituted cysteines are compared to cysteine hydrochloride as color reagents. The accuracy of the modified procedure is about ± 2% in the optimum concentration range. The time required for a complete analysis is about 1 h for pentoses and hexuronic acids and 30 min for hexoses.
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