Study of subchronic toxicity of Primalact preparation at intracisternal administration

Abstract

Currently, the problem of treatment and prevention of mammary gland diseases in cows is relevant. In this regard, the task of developing new drugs to combat mastitis, with a wide spectrum of antimicrobial activity and minimal side effects, remains relevant. Authors studied subchronic toxicity of Primalact preparation with intracisternal administration to lactating cows. The experiment was performed on 12 clinically healthy cows at the 3rd-4th months of lactation. 3 experimental and 1 control groups were formed. Animals in the experimental groups for 9 days were intracisternally injected in doses of 5, 10 and 20 ml, respectively. Examination data showed the absence of significant changes in the clinical state of the experimental animals and breast tissue in the two experimental groups. In the group with a dose of 20 ml on the 5th day, an increase in the temperature of the udder lobes, their densification, slight pain, the appearance of flakes in the secretion of the udder and a decrease in dairy productivity were detected. In this case it indicated the presence of an irritating effect. The administration of Primalact for 9 days in a dose of 5 ml and 10 ml to lactating cows did not cause any changes in the morphological and biochemical blood parameters. The results of the studies in the fourth experimental group were reflected in the instructions for the application of the drug, which indicated that the frequency of administration of the drug was 3-4 days. Thus, Primalact, when intracisternially administered to lactating cows in doses of 5 and 10 ml for 9 days, does not have subchronic toxicity.