Abstract
Applications of high performance liquid chromatography on a gel exclusion column (HPLC-GEC) for the characterization of liposomes are presented following a review of the conventional uses of gel exclusion chromatography (GEC). The practical aspects of HPLC-GEC concerning the choice of the gel and the column operation are examined. A procedure for column calibration with vesicles is proposed, in order to obtain the selectivity curve of the TSK-G6000 PW gel; this appears to be the most convenient gel for sizing of liposomes. The mechanism of elution is discussed as a function of the particle size and composition. Methods involving this type of column and different detection modes (light scattering, refractive index, fluorescence and radioactivity) or their combination, were investigated in detail either to analyse the polydispersity of liposomes or their size stability in terms of aggregation and/or fusion.
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