Study of single nucleotide polymorphisms and serum level of E-cadherin and their relation to psoriasis severity

Abstract

Backgroundpsoriasis is a persistent autoimmune-mediated systemic inflammatory illness that affects the skin and several organ systems. A family of calcium-dependent cell adhesion molecules called cadherins (CDH) works through homophilic contacts to keep cells attached to one another. In comparison to normal skin, several investigations found that the expression of E-cadherin was downregulated in the granular and basal layers of psoriasis lesional skin. ObjectivesThis study aimed to relate the serum level of E-cadherin and its genes (rs17715799 and rs13689) SNPs with the severity of psoriasis. MethodsIn the current study, 120 participants were divided into two groups: 60 patients with plaque psoriasis were in group I, and 60 healthy controls were in group II. The serum level of E-cadherin protein was measured using an enzyme linked immunosorbent assay (ELISA), and SNPs in the E-cadherin gene (rs17715799 and rs13689) were identified using real-time polymerase chain reaction (RT-PCR) technology. ResultsIn our study group I had a considerably higher serum level of the protein E-cadherin. It was confirmed that the E cadherin (rs13689) genotype frequency distribution revealed a significant difference between the two studied groups. The frequency of the CC genotype was higher in group I. While there was no significant variation in the genotype frequency distribution of E-cadherin (rs17715799) between the two study groups under the dominant, over-dominant, and recessive modes of inheritance (p 0.204) conclusion: patients with psoriasis had considerably higher serum levels of E-cadherin. The E-cadherin gene rs13689 polymorphism affects the chance of developing psoriasis. However, the E-cadherin gene rs17715799 polymorphism has no impact on the onset of psoriasis. As a result, the E-cadherin gene rs13689 but not rs17715799 might be considered as a marker for the risk of developing psoriasis.