Study of Serum Adenosine Deaminase (ADA) Activity in Pulmonary Tuberculosis Patients of Indian Subpopulation

Abstract

Background Adenosine deaminase ADA activity has largely been studied using sputum and effusion fluids for diagnosing tuberculosis TB in the affected patients. However it is not feasible to access these specimens for continuous monitoring. The purpose of this study was to monitor the activity of ADA using serum in pulmonary TB PTB patients.Objectives The study was proposed to estimate the levels of serum ADA in PTB patients and to correlate these levels with normal healthy individuals.Methodology A total of 60 participants comprising of thirty subjects with PTB and thirty healthy subjects with age ge18 years were included in the study. Blood samples were collected from the antecubital vein from each participant and serum samples were used for the estimation of ADA levels using enzymatic method.Result Mean serum level of ADA in PTB patients was 12.6plusmn5.1 UL and control group was 3.2plusmn2.2 UL. PTB patients showed significant elevated levels of serum ADA p lt0.001.Conclusion Serum ADA levels significantly increased in PTB patients compared to healthy individuals. The biological reference interval obtained in the study was different from the one provided by the manufacturer.nbsp