Study of Selenium Binding Protein Inside Erythrocytes

Abstract

When selenium is added to human blood in vitro it is taken up by the erythrocytes in the first minutes and then most of which is released into the plasma. By using the technique of gel filtration chromatography (Sephadex G-75) for separating the selenium binding protein inside erythrocytes, it was found one protein peak for sample treated with selenium for (5, 30 min) and sample without selenium. It is clear that maximum concentration of selenium was found in protein peak treated with selenium for (5 min), while protein peak treated with selenium for (30 min) showed a higher molecular weight. The results demonstrate the binding of selenium with protein inside erythrocytes and its release into the plasma leaving the selenium binding protein.