Study of S. aureus isolated from wound infection of Rhinoplasty

Abstract

A total of 110 wound swabs were collected from the beginning of February 2020 to the end of April 2021 from patients attending private plastic surgery clinics. Collection of vaginal swabs included cultivation on blood agar and MacConkey agar for 24 hour to evaluate the role of S aureus in surgical infection associated with elective rhinoplasty. Media were organized and purified by the producer's direction. The prepared media were used for separation, affirmation of the useful check, conspicuous confirmation and weakness testing these media were finished in the wake of being solidified. Swabs was inoculated onto MacConkey, supplement and blood agars. By then the vaccinated plates were brought forth at 37°C for 24 hr. Inoculum from the attempted bacterium was prepared. A single territory was moved to a by sterile q-tip is dove into the inoculum and subsequently cleaned consistently over the outside of a Muller-Hinton agar plate, after that inside 15 minutes of inoculation, the antimicrobial-containing circles are applied to the agar with a forceps crushed determinedly to ensure contact with agar and a while later plate switched and brought forth at 37oC for 18 hours. According to distribution of the positive culture, the highest percentage of positive wound culture were within the age group 16-25 year (37.04%), followed by 25-35 yea (25.93%). According to the distribution of the isolated bacteria among the study groups, the common isolated bacteria from wound infection was S. aureus with rate (62.96%), followed by E.coli(14.81%) and the lowest rate was with K. pneumoniae, Pseudomonas aeruginosa and S epidermidis. Regarding to the virulence factors of Staphylococcus aureus, the study showed that, all S. aureus isolates were coagulase and DNase positive, 94.11% of isolates were beta-hemolysis, 88.23% were characterized by invasiveness (Growth on congored Agar), 82.35% of isolates was lecithinase and capsule production while 29.41% were protease production with highly significant relation between Staphylococcus aureus and in wound infection. the high rate of vancomycin resistance as virulence factors was found among S. aureus isolates (11.76%). Beta-lactam and methicillin resistances was recorded highly among S. aureus isolates (70.58%)