Abstract
Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogens and contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rust resistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversity and Nei and Li's coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair Group Method of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereas minimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways to produce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improved characteristics.
Highlights
Wheat is the most important staple diet all over the world
It is known to be evolved from wild timopheevi wheat and is the only Lr gene located on the long arm of wheat homoeologous group 2 chromosomes
KSUD14 has the structure of a resistance gene analog (RGA) including kinase2a and kinase3 domains similar to the Cre3 gene of wheat and the rust resistance gene Rp1-D of maize [9]
Summary
Wheat is the most important staple diet all over the world. It is internationally an important commodity for marketing. Pakistan and India contribute 20% in annual world wheat production. The characterization of wheat rust resistance genes is necessary to control the disease so that we could incorporate these genes into the germplasm of elite varieties [2]. Molecular markers brought forward efficient methods of plant breeding. Many new races of leaf, stripe and stem resistance have been known to evolve and showed threat for available rust resistance genes. Various studies on wheat have been carried out using molecular marker system to characterize gene of choice, mapping of qualitative trait loci and genetic diversity analysis. This allows the marker assisted selection to occur.
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