Abstract
Abstract By applying the in-silico method, resveratrol was docked on those proteins which are responsible for bone loss. The Molecular docking data between the resveratrol and Receptor activator of nuclear factor-kappa-Β ligand [RANKL] receptors proved that resveratrol binds tightly to the receptors, showed the highest binding affinities of −6.9, −7.6, −7.1, −6.9, −6.7, and −7.1 kcal/mol. According to in-vitro data, Resveratrol reduced the osteoclasts after treating Marrow-Derived Macrophages [BMM] with Macrophage colony-stimulating factor [MCSF] 20ng / ml and RANKL 50ng / ml, with different concentrations of resveratrol (2.5, 10 μg / ml) For 7 days, the cells were treated with MCSF (20 ng / ml) and RANKL (40 ng / ml) together with concentrated trimethyl ether and resveratrol (2.5, 10 μg / ml) within 12 hours. Which, not affect cell survival. After fixing osteoclast cells with formaldehyde fixative on glass coverslip followed by incubation with 0.1% Triton X-100 in PBS for 5 min and after that stain with rhodamine phalloidin staining for actin and Hoechst for nuclei. Fluorescence microscopy was performed to see the distribution of filaments actin [F.actin]. Finally, resveratrol reduced the actin ring formation. Resveratrol is the best bioactive compound for drug preparation against bone loss.
Highlights
Medicinal plants are very rich in phenolics and many bioactive compounds and have the potential for the synthesis of natural drug (Arcanjo et al, 2012; Sabir et al, 2021)
Bone Marrowderived Macrophage (BMM) isolated from Sham and Ovariectomy mice were treated with cytokines like M.CSF and Receptor Activator of Nuclear Factor kappa-B Ligant (RANKL) along with Resveratrol
Our findings showed that bone loss is because of RANKAL proteins
Summary
Medicinal plants are very rich in phenolics and many bioactive compounds and have the potential for the synthesis of natural drug (Arcanjo et al, 2012; Sabir et al, 2021). There is a rich family of complementary nutrients, especially in the case of monomeric trihydroxystylbenzene resveratrol (3 5 4’-trihydroxy-trans-stilbene), which has major components that affect the effects of cardiovascular protection in red wine (Baur and Sinclair, 2006; Sousa et al, 2006). Molecular Docking is an effective and qualitative tool for in-silico examinations It plays an important role and has increased proportions of reasonable drugs (Drews, 2000). Docking is a calculation procedure for finding a suitable ligand that fits very well and geometrically in areas of binding proteins. In other words, it is a study of how two or more molecules, eg. The software analysis of the binding analogue may be used as a predictive tool for the design of novel therapeutic compounds concerning the blocking of CYP3A4 and to facilitate the detection of biochemical nature of the interaction of dietary components, Plant and pharmaceutical compounds that are facilitated by the enzyme (Basheer et al, 2015)
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