Abstract
Study of reproduction cycles and yield dynamics of the classical swine fever virus in PK-15 cultured cells using a fluorescent probes technique
Highlights
The classical swine fever (CSF) caused by the homonymous RNA-containing virus (CSFV), a member of Pestivirus genus (Flaviviridae family), is still among the most dangerous swine infections
In some countries belonging to the former Soviet Union several anti-CSFV vaccines have been elaborated and are produced containing vaccine virus strains
To cons truct any up-to-date antiviral vaccines aimed for the CSF prevention, a biotechnological problem of the large scale CSFV cultivation followed by a high virus yield is to be successfully solved
Summary
The classical swine fever (CSF) caused by the homonymous RNA-containing virus (CSFV), a member of Pestivirus genus (Flaviviridae family), is still among the most dangerous swine infections. The widest investigations concerning recombinant vaccine construction are carrying out. Such vaccines are not yet completely studied. To cons truct any up-to-date antiviral vaccines aimed for the CSF prevention, a biotechnological problem of the large scale CSFV cultivation followed by a high virus yield is to be successfully solved. The solving of this problem, is almost impossible without full information concerning reproductive cycles of both vaccine and virulent CSFV strains; such information is to be obtained using virus-sensitive cell culture infection
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