Abstract
Along with the wide distribution and use of alfalfa grass there is no comprehensive scientific pharmacognosy studies about representatives of the plants of this kind. Aim. To study the content of the group of true coumarins and 4-oxyderivatives which presence must be characteristic for the yellow alfalfa. Materials and methods. The determination of diagnostic anatomical features of the plant Medicago falcata L. subsp. romanica (Prodan) O. Schwarz & Klink has been carried out. The leaf petiole has such diagnostic characteristics: elongated epidermal cells, parenchymal or prosenchymal, 4-, or (rarely) 5-6 gonal; pubescence is of simple single-celled hairs with warty cuticle, which can be found on the upper and lower epidermis of the leaf. The stomatas are rare. The rosette is formed from basal epithelial cells near the base of the hairs. Results. 66 Components have been revealed by chromatography of native raw, 38 of components have been identified, namely - dihydrocoumarin (1.60%) and coumarin (13.13%). After the acid hydrolysis 52 components have been revealed, 41 of which have been identified. These substances are the complex of biologically active compounds: primary alcohols, aldehydes, ketones. 4-Oxycumarin derivatives have been identified: coumarin - 79.38% dihydrocoumarin - 4.95%, and 6-methylcoumarin - 0.39%. While studying the amino acid composition of the above-ground parts of alfalfa yellow we got interested in a significant amount of aspartic acid, proline and phenylalanine.
Highlights
При великому поширенні та використанні трави люцерни відсутні наукові комплексні роботи з фармакогностичними дослідженнями представників рослин цього роду
To study the content of the group of true coumarins and 4-oxyderivatives which presence must be characteristic for the yellow alfalfa
66 Components have been revealed by chromatography of native raw, 38 of components have been identified, namely dihydrocoumarin (1.60%) and coumarin (13.13%)
Summary
Мета роботи – вивчення вмісту груп сполук істинних кумаринів та їхніх 4-оксипохідних, наявність яких має бути характерною для люцерни жовтої (серпоподібної або румунської). Визначали діагностичні анатомічні ознаки рослини Medicago falcata L. subsp. Виявили при хроматографуванні нативної сировини 66 компонентів, з яких ідентифікували 38 речовин. Серед них – дигідрокумарин (1,60%) та кумарин (13,13%). Після здійснення кислотного гідролізу виявили 52 компоненти, з них 41 речовину ідентифікували. Виявили похідні 4-оксикумарину: кумарин – 79,38%, дигідрокумарин – 4,95% та 6-метилкумарин – 0,39%. Цель работы – изучение содержания групп соединений истинных кумаринов и их 4-оксипроизводных, присутствие которых должно быть характерным для люцерны жёлтой (серповидной или румынской). Выявлено при хроматографировании нативного сырья 66 компонентов, из которых идентифицировано 38. Среди них – дигидрокумарин (1,60%) и кумарин (13,13%). To study the content of the group of true coumarins and 4-oxyderivatives which presence must be characteristic for the yellow alfalfa
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