Study of Pu-erh tea extract on inhibiting proliferation and inducing apoptosis of tongue squamous cell carcinoma Tca8113 cell in vitro

Abstract

Objective To study inhibition effect of Pu-erh tea extract (PTE) on tongue squamous cell carcinoma Tca8113 cell and its mechanism.Methods The inhibition on proliferation and growth of Tca8113 cell and periodontal ligament fibroblast (PDL) was detected with methyl-thiazol-tetrazolium method.The morphological changes of cells were observed under inverted phase contrast microscope.The mechanism of inhibition was studied using flow cytometry.The data were analyzed with GraphPad Prism 5.01 software and compared by one-way ANOVA.Results Tca8113 cell viability decreased clearly with the increase of PTE concentration.When the concentration of PTE was 125.00 μg/ml,the most significant decrease was observed (F=1 283,P＜0.000 1).Tca8113 cell viability decreased with the increase of time with the most significant difference at 12 h (F=111.6,P＜0.000 1).However,the growth and proliferation of PDL were not influenced by PTE.Shrinkage,roundness and detachment were observed in Tca8113 cell but not in PDL under microscope.Apoptosis rate was 27.65％ ±0.47％ after Tca8113 cells were treated with 125.00 μg/ml PTE for 12 h.Conclusion PTE can inhibit Tca8113 cell proliferation by inducing apoptosis,suggesting that it could prevent or treat tongue squamous cell carcinoma. Key words: Tea; Tongue neoplasms; Fibroblasts; Apoptosis