Abstract
Abstract Investigators studying mechanisms of cell regulation often need to establish that certain proteins are phosphorylated in intact cells. The usual technique employed for these studies is to label the cellular ATP pools with 32P prior to applying a stimulus that is expected to change the phosphorylation state of certain proteins or sets of proteins. The phosphorylated proteins are extracted from the cell, resolved on SDS-polyacrylamide gels, and visualized using autoradiography.
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