Abstract
One of the main etiological factors in the development of some malignant tumours is the presence of chronic inflammatory processes. A number of biomedical studies have confirmed the anticarcinogenic effect of anti-inflammatory drugs with respect to colon cancers. In addition, some biologically active substances present in medicinal plants have been found to exhibit both anti-carcinogenic and antioxidant activity. In this paper, we investigate potential anticarcinogenic effects of extracts obtained from plant raw materials, including lingonberry (Vaccinium vttis-idaea), raspberry (Rubus idaeus), black chokeberry (Aronia melanocarpa), grape seeds (Vitis L.), grape pomace (Vitis L.), Krasnodar green tea (Camellia sinensis), ginseng (Panax L.), fireweed (Chamaenerion angustifolium) and coffee (Coffea arabica). The effect of these extracts on the growth and viability of colon HCT-116 cancer cells was assessed in vitro using the method of MTT assay. In addition, the effect of non-toxic doses of the extracts under study on the basal and induced levels of pro-inflammatory gene expression - cyclooxygenase (COX2), inducible NO synthase (iNOS) and interleukin 8 (IL8) - was assessed by quantitative PCR. The working concentrations of the extracts under study for HCT-116 cells were determined. It is demonstrated that all investigated extracts are capable of suppressing the expression of COX-2, iNOS and IL-8. Black chokeberry and fireweed extracts exhibit the most pronounced total inhibitory effect on the expression of these inflammatory genes. Future research should investigate effects of black chokeberry and fireweed extracts on the induced expression of COX-2, iNOS and IL-8 and their anti-carcinogenic activity in vivo. The antioxidant activity determined using DPPH and FRAP was the highest for black chokeberry, raspberry and fireweed. A relationship between the anticar-cinogenic and antioxidant effects of the extracts under study was revealed.
Highlights
One of the main etiological factors in the development of some malignant tumours is the presence of chronic inflammatory processes
The anticancer effect of poly- gold nanoparticle Coumarin-gold nanoparticle biophenols against breast cancer and cancer stem cells: conjugates: preparation, antioxidant, and cytotoxic molecular mechanisms // Nutrients. 2016
Lage N.N., Layosa M.A.A., Arbizu S., Chew B.P., Pedrosa M.L., Mertens-Talcott S., et al Dark sweet cherry (Prunus avium) phenolics enriched in anthocyanins exhibit enhanced activity against the most aggressive breast cancer subtypes without toxicity to normal breast cells // Journal of Functional Foods. 2020
Summary
Обратная дозозависимость (максимальное подавление экспрессии COX-2 при минимальной концентрации) была продемонстрирована для экстрактов зеленого чая (6) и иван-чая (8). Наиболее выраженно этот эффект наблюдали в случае экстрактов черноплодной рябины (3), выжимок винограда (5) и иван-чая (8). Наиболее выраженным суммарным ингибирующим действием на экспрессию данных генов обладали экстракты черноплодной рябины (3) и иван-чая (8). Проведенные анализы по радикалудерживающей способности методом DPPH выявили, что лидером являются экстракты черноплодной рябины (1,0 мг/см3), малины (2,0 мг/см3), семян винограда (3,1 мг/см3) и иван-чая (4,0 мг/см). В случае определения железосвязывающей способности по методу FRAP определено, что наибольшей силой обладают также экстракты женьшеня (10,19 ммоль Fe2+/кг), черноплодной рябины (18,2 ммоль Fe2+/кг), иван-чая (22,14 ммоль Fe2+/кг) и малины (22,3 ммоль Fe2+/кг). Во всех показателях наибольшую антиоксидантную активность проявляют одини и те же экстракты, а именно, экстракты черноплодной рябины, малины и иван-чая, что коррелируются с данными по антиканцерогенной активности
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