STUDY OF POLYMORPHISM OF RED CURRANT VARIETIES MICROSATELLITE LOCUS

Abstract

In the presented studies the genotypes of 46 varieties of red currant (Ribes rubrum) from the All-Russian Research Institute of Fruit Crops Breeding (VNIISPK) the collection were studied for 14 microsatellite locus. The data were obtained by the detection method of – fragment analysis using capillary electrophoresis. Varieties with rare, unique alleles and combinations of alleles have been identified. In most cases, no more than two fragments were identified for each genotype for a specific locus, but three fragments at some loci were amplified in the samples (Orlovchanka, Konstantinovskaya, Nadezhda – e3-B02; Marmeladnitsa – g2-G12; Nadezhda – g2-J08; Svetlitsa, Tatianina – g1-L12). The profiles of the cultivars Cascad and Red cross are identical to each other, although they differ from the others. The Red cross [(Cherry × White Grape] and Cascad [(free pollination of the variety Diploma (Cherry × White grape)]] varieties were developed in the USA and have common ancestors. It can be assumed the collection under different names contains the same genotype or that these pairs of varieties are so similar that the polymorphism of the analyzed loci is not enough to distinguish them. To prevent a shift in the size of alleles control varieties were selected and proposed, zoned in the territory of the Russian Federation – Gazelle (originator of VNIISPK: Chulkovskaya × Maarses Prominent) and Valentinovka (originator of VNIISPK: Rote Shpetlese × Jonker van Tets). The data obtained on the polymorphism microsatellite loci Red currant can be used for further identification.