Abstract
Polydiacetylene (PDA) is an attractive conjugated material for use in biosensors due to its unique characteristic of undergoing a blue-to-red color change in response to external stimuli. 10,12-Pentacosadiynoic acid (PCDA) and poly (ethylene oxide) (PEO) were used in this study to develop fiber composites via an electrospinning method at various mass ratios of PEO to PCDA, solution concentrations, and injection speeds. The PEO-PDA fibers in blue phase were obtained via photo-polymerization upon UV-light irritation. High mass ratios of PEO to PCDA, low polymer concentrations of spinning solution, and low injection speeds promoted fine fibers with small diameters and smooth surfaces. The colorimetric transition of the fibers was investigated when the fibers were heated at temperatures ranging from 25 °C to 120 °C. A color switch from blue to red in the fibers was observed when the fibers were heated at temperatures greater than 60 °C. The color transition was more sensitive in the fibers made with a low mass ratio of PEO to PCDA due to high fraction of PDA in the fibers. The large diameter fibers also promoted the color switch due to high reflectance area in the fibers. All of the fibers were analyzed using Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) and compared before and after the color change occurred. The colorimetric transitional mechanism is proposed to occur due to conformational changes in the PDA macromolecules.
Highlights
When biomaterials are analyzed using biosensors, biological responses can be converted by the biosensor into measurable signals, providing analytic tools for the compositions, structures, and functions of the biomaterials [1,2]
PEO-PDA fibers in nano and submicron size were obtained on a flat collector placed at a distance of 17 cm
When the mixture of PEO and Pentacosadiynoic acid (PCDA) was stretched during the electrospinning, there were larger attractive forces between the PCDA monomers than those between the PCDA and the PEO matrix, resulting self-assemblies of the PCDA monomers in the obtained fibers
Summary
When biomaterials are analyzed using biosensors, biological responses can be converted by the biosensor into measurable signals, providing analytic tools for the compositions, structures, and functions of the biomaterials [1,2]. Ideal biosensors can provide short response time, high precision and accuracy, and painless diagnosis in both in vitro and in vivo applications including infectious disease monitoring, food safety, environmental monitoring, and military biodefense [3]. One promising application of biosensors is to detect pathogens and bacterial infections in hygiene processes, such as wound care and personal care. Current wound care management of pathogen infection may be time-consuming because it usually requires multiple steps including physical examination, imaging of the wound, and sample testing, sometimes resulting in a delay of treatment [4]. Such a delay could be life-threatening in chronic wound management. Conjugated polymers have gained interest in biosensor development because they exhibit reliable bio-sensing activities, which are usually associated
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
