Abstract
Abstract: This study is an evaluative effort to analyse different physiological parameters and rheological properties observed during the fermentative production of cyclosporin A. Tolypocladium inflatum culture was used for seed development. For production fermentation, the mature seed was inoculated at specific transfer parameters. At each stage of the current study, pH, PMV and sugar consumption were keenly analysed and recorded. Different aeration rates and agitation speeds were evaluated during various stages of growth. Based on several experiments, the most optimised parameters were included for further analysis. The maximum productivity obtained was 8.3 g/L. Further, different kinds of solvents were studied to extract crude from the fermentation broth. Solvents required at various stages of downstream processing were analysed for best recovery with minimal product loss. Finally, the product was obtained with 80.58% recovery. Keywords: cyclosporin A, Tolypocladium inflatum, fermentation, seed, pH, PMV, extraction, downstream processing
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