Study of physicochemical properties of Indrayava (Holarrhena antidysenterica wall.) and its antibacterial effect on Enteropathogenic e-coli (EPEC) (in vitro)

Gawhare Vikesh S

doi:10.47552/ijam.v4i2.264

Abstract

As stated in Ayurvedic texts Indrayava mainly useful in treatment of diseases like diarrhoea, dysentery etc. E-coli is most dangerous bacteria causing diarrhoea. Ayurveda has included all the microbes under the heading ' Krimi '. As per Bhavaprakasha Nighantu , Indrayava having Atisaraghna and Krimighna property. Hence Indrayava having action on bacteria ( Krimi ) may have action on E.coli causing diarrhoea (EPEC). So it is necessary to do the physicochemical standardization of Indrayava , to study its antibacterial activity on EPEC (In-vitro), to determine minimum inhibitory concentration of Indrayava for antibacterial activity against EPEC. Materials used are self collected sample, clinically isolated EPEC. Method used for antibacterial susceptibility is disc diffusion method. After study result came are, foreign matter is negligible, moisture content is 8.57%, total ash is 4.61%, acid insoluble ash is 0.60%, water soluble ash is 6.67%, water soluble extract is 33.32%, alcohol soluble extract is 30.51% and pH is 5.42. Indrayava shows the antibacterial activity against Enteropathogenic E-coli (EPEC) in methanolic extract having minimum inhibitory concentration value 2.0gm/10ml.

Highlights

Ayurveda is the flawless ancient science of life, the word ‘Ayur’ literally means ‘life’ and ‘Veda’, the ‘science’ or ‘knowledge’
From the discussion it is concluded that the self collected sample of Indrayava is standard with respect to its pharmacognostical, physicochemical, phytochemical test
Antibacterial study with reference to E.coli shows the positive results in methanolic extract of Indrayava

Summary

Introduction

Ayurveda is the flawless ancient science of life, the word ‘Ayur’ literally means ‘life’ and ‘Veda’, the ‘science’ or ‘knowledge’. This system of medicine is based on holistic approach and origin of it can be traced to as early as dawn of the civilization and Vedic period. Its aim is not just the cure of disease but the maintenance of a positive healthy state of body, mind and spirit in a healthy environment and in harmony with the universe.

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Conclusion