Abstract
Langmuir-Blodgett (LB) films of bacteriorhodopsin (BR) in soya-phosphatidylcholine (PC) matrix were prepared on quartz plates and the photocycle and spectral properties of the films were studied. The transient absorption spectrum of BR LB film was measured by the nanosecond laser flash photolysis method in a wavelength range from 380 to 650 nm. The absorption maxima were at 540 and 415 nm for the L and M states respectively. In water the rate of decay of the L state was 2.3 × 10 4 s −1 and the rates of formation and decay of the M state were 2.3 × 10 4 s −1 and 3.0 × 10 2 s −1 respectively. The photocycle reaction was not observed in nitrogen-dried BR LB films, but when the films were put back in water, even after being stored for 8 months in dry conditions, the phenomenon could be observed. The energy transfer from trytophan to BR retinal takes place in both dry and wet LB films. The films were relatively stable to changing physical conditions as well as to laser excitation and no changes were observed following repeated photocycles. Molecular surface potentials of BR-soya-PC and soya-PC Langmuir films as a function of mean molecular area decreased towards zero with increasing surface pressure.
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