STUDY OF PHENOTYPIC AND GENOTYPIC PATTERNS OF Escherichia coli ISOLATED FROM EUPHRATES RIVER IN RAMADI CITY

Abstract

The present study was preformed in period between October 2011 to July 2012, isolated were isolated from Euphrates river in Ramadi city . samples were cultured on several culture media microscopical, cultural, biochemical,serotypical (by use of serotype Diagnosis kit of bacteria ) and genotypical (eae , stx , bfp ) diagnosis of bacteria were done . The results showed that 278 from Euphrates samples were E.Coli ( 68.137 %) also the results indicated that (28) isolates belong to EPEC and the dominant serotype were type1 multisampling equivalent. with ratio of (39.28%) followed by the multi-samples equivalent type4 (25%) and the multisampling equivalent type2 and type2 with a ratio of (17.85%) for each one .In addition the mono sample equivalent serotype ratio was O55 ( 21.42%) followed by 0142 with a ratio of (14.28%). Then , the sample typing 088 and 0126 come with a ratio of (10.7 %) . All serotype patterns were tested to check the content of virulence determinant and the results showed that 19 isolates contained the gene eae ratio( 67.85%) ,when as there is no any isolates contained bfp , stx genes EPEC bacteria also disinfected to typical EPEC and Atypical EPEC , where the typical EPEC were found in 28 isolates and Atypical EPEC identified in 19 isolates with the ratio (67.85%) .