Study of Oxidative Stress Marker 8-hydroxy-2’-deoxyguanosine in Chronic Kidney Disease Patients

Abstract

Background and aim Renin-Angiotensin system has been implicated in pathological changes of organ damage through modulation of gene expression proliferation and inflammatory response. The aim of this study was to identify the role of angiotensin-converting enzyme ACE gene alleles with respect to treatment and effect in chronic kidney disease CKD patients. The objectives of the study were to determine the role of ACE gene allele in haemodialysis treatment of CKD and the effect of ACE gene on 8-hydroxy-2 -deoxyguanosine 8-OHdG level in CKD.Methods The patients available in the medicine department diagnosed with CKD for a period of 12-15 months were included in the study. The peripheral venous blood was used for DNA isolation as per the Quigen kit. The isolated DNA was amplified by polymerase chain reaction PCR using a primer for ACE gene as per the protocols defined previously. The PCR product was subjected to electrophoresis for detection of insertion and deletion. The venous blood was also subjected to 8-OHdG enzyme-linked immunosorbent assay ELISA assay for oxidative damage analysis.Results The mean plusmn SD values of ACE gene alleles subject to the number of haemodialysis were found to be 185.82 plusmn 131.69 in DD allele 339.33 plusmn 261.59 in II allele and 183.33 plusmn 182.93 in DI allele. The mean plusmn SD values of ACE gene alleles subject to serum 8-OHdG level were found to be 39.02 plusmn 29.5 in DD allele 15.74 plusmn 11.76 in II allele and 25.39 plusmn 23.66 in DI allele respectively.Conclusion The number of hemodialysis and oxidative stress marker level differences were statistically insignificant plt0.05 in patients with different ACE gene alleles.