Abstract
The present work involved study of wastewater contaminant removal along with EPS production by a thermotolerant bacterium Bacillus sp. ISTVK1, isolated from heat shocked sewage sludge. EPS production in basal and mineral medium containing 50% filter sterilized wastewater and 0.5% sucrose was found to be 0.83±0.12gL−1 and 0.31±0.10gL−1 culture, respectively. GC–MS analysis of EPS revealed the presence of β-d-glucose, α-d-galactose and β-d-arabinose. FT-IR spectrum confirmed the presence carbohydrates. Box–Behnken design was used to optimize process parameters for enhanced EPS production along with % COD reduction of wastewater. The optimised conditions when used in a 1.5L bioreactor showed EPS production of 1.67±0.06gL−1 culture and 93.0±0.21 % COD removal.
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