Abstract
Study of Optimal Real-time Polymerase Chain Reaction Chip by Comparison of Coating Reflective Materials
Highlights
The polymerase chain reaction (PCR) is a molecular biology technique that amplifies small amounts of genetic samples in large quantities
When we visually compared several printed circuit board (PCB) substrates printed with silk legends produced by the PCB manufacturer, we found that the white silk color and density deviations were very large
To select the optimal chip material, subtract the average brightness of the chip that was injected with distilled water from the Relative gains of the four types of real-time polymerase chain reaction (RT-PCR) chips
Summary
The polymerase chain reaction (PCR) is a molecular biology technique that amplifies small amounts of genetic samples in large quantities. This technique is used to amplify nucleic acids such as DNAs or RNAs to diagnose various genetic diseases. It can be applied to bacteria, viruses, and fungi to diagnose infectious diseases. Since the existing PCR technique is unable to determine the success of the amplification by only amplifying the DNAs or the RNAs, it was necessary to perform an electrophoresis test to verify the amplification of the DNA. Since additional experiments after the PCR test are required, is the entire experiment time prolonged, and contamination may occur during the electrophoresis.
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