Abstract
This paper describes the analysis of marbofloxacin at low levels in pig plasma by capillary electrophoresis (CE). To increase the sensitivity of CE we studied two on-column stacking systems, field-amplified sample injection (FASI) and isotachophoresis (ITP), and optimized several conditions to obtain the best sensitivity with each. The best results were obtained by use of ITP-CZE with 10mm sodium monohydrogen phosphate (pH 9.0) as the leading and background electrolytes and 10mm β-alanine (pH 9.0) as the terminating electrolyte. This system was 75 times more sensitive than conventional CE—the enhanced detection limit (LOD) was 20 μg kg−1. A sample of pig plasma was analyzed after clean-up by solid-phase extraction on Oasis HLB cartridges and elution with methanol. Recoveries were approximately 100%.
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