Abstract
To investigate the effects of nano-graphene oxide on the number of kupffer cells and megakaryocytes, in vivo method was applied. In this study, four groups of livers including control, sham, experimental group 1 (using a dose of 17 mg/kg), experimental group 2 (using a dose of 5.5 mg/kg), were investigated. On day 9 of gestation, control group without the effect of graphene oxide, sham group with injection of water as graphene oxide solvent and experimental groups with injection of graphene oxide (1.2 nm particles) with doses of 17 and 5.5 mg/kg mouse weight were examined. Then, on day 15 of gestation, embryos were removed from the mother`s body and their livers were amputated. The statistical results obtained by counting the number of kupffer cells and megakaryocytes in experimental groups that received nano graphene oxide, showed significant changes as compared with the sham and control groups. In the dose of 17 mg/kg there was a significant increase (P<0.001) in the number of kupffer cells and significant increase in the dose of 5.5 mg/kg (P<0.05) in the number of megakaryocytes. These findings showed the destructive effect of nano-graphene oxide on the development of liver in the condition of in vivo.
Highlights
More rapid development of nanotechnology led to entering nanomaterials into human life, the environment or ecosystem (Murr et al.,2004)
The main role of kupffer cells is phagocyte foreign materials, safety, supervision and regulation of physiological homeostasis of liver.Megakaryocyte is large nuclear cells in bone marrow which produces blood thrombocytes, which are essential for normal blood clotting (Branehog et al.,1975) Megakaryocytes are from hematopoietic stem cells (HSCs) which mainly created in the bone marrow, and be in the yolk sac, fetal liver and spleen during early development [6,7,8]
We observed significant differences between all studied cases, By comparing histograms (Figures 3 and 4) of each of the samples together (* P
Summary
More rapid development of nanotechnology led to entering nanomaterials into human life, the environment or ecosystem (Murr et al.,2004). The main role of kupffer cells is phagocyte foreign materials, safety, supervision and regulation of physiological homeostasis of liver.Megakaryocyte is large nuclear cells in bone marrow which produces blood thrombocytes (platelets), which are essential for normal blood clotting (Branehog et al.,1975) Megakaryocytes are from hematopoietic stem cells (HSCs) which mainly created in the bone marrow, and be in the yolk sac, fetal liver and spleen during early development [6,7,8]. Graphene due to its similar chemical structure to carbon nanotubes can be used as carriers in pharmaceuticals (Zhuang et al.,2011) Carbon nanotubes and graphene are two types of carbon materials of sp bonded with small dimensions, these materials based on showing special physical and chemical properties, attracted to a wide range of sciences, including Nanomedicine pharmaceuticals (Zhuang et al.,2011).GO and graphene in recent years emerges as a promising material for many new applications in nanoelectronics
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
