Study of Mycobacterium bovis genotypes in human and bovine isolates using spoligotyping, MIRUVNTR and RFLP-PCR

Abstract

Aims and objectivesThe aim of this study is to investigate and detect the prevalence of Mycobacterium bovis subtypes (Mycobacterium bovis subtype bovis and Mycobacterium bovis subtype caprae) in humans and compare the genetic diversity of Mycobacterium bovis in humans and cattle with spoligotyping methods, as well as pyrazinamide susceptibility study of subtypes. MethodsExamining these subtypes with molecular epidemiology techniques is particularly important due to different treatment of M. bovis diverse subtypes in humans. Culture tests were performed on clinical samples that were isolated from Lowenstein-Jensen culture medium. Identification tests were performed to differentiate Mycobacterium bovis from Mycobacterium tuberculosis. DNA was extracted and spoligotyping (spacer oligonucleotide typing) was performed using the DRb and DRa primers. ResultsThe results were analyzed with the SPOLDB4 site. PCR-RFLP method of pncA gene was used to evaluate the resistance to pyrazinamide and pncA gene polymorphism. ConclusionsMycobacterium bovis subtype bovis in the Iranian population was reported with a frequency of 0.6% which was below the average of the previous reviews. In this study, all the strains were M. bovis subtype bovis resistant to pyrazinamide. No Mycobacterium bovis subtype caprae was detected. The only shared ST between humans and cattle was ST694. Mycobacterium bovis subtype bovis with ST 595 was reported as human bovis index.