Study of micronutrient status of patients with obesity and diabetes mellitus type 2 based on molecular genetics analysis

Abstract

Introduction. Type 2 diabetes mellitus (type 2) is a major health problem worldwide. The progression of type 2 diabetes is contributed by usual for this patient group number of micronutrient deficiencies.Aim. To study the micronutrient status in patients with type 2 diabetes and obesity on the basis of molecular genetic studies.Materials and methods. The study involved 80 women with type 2 diabetes and obesity of I-II degree. All patients were tested for vitamin C, B6, B12, folate, a 25-hydroxyvitamin D, as well as potassium, magnesium, calcium, zinc and phosphorus in serum. DNA was isolated from blood by standard method using multicomponent lysis solution. Genotyping was performed using allele-specific amplification detection results in real time.Results. Comparative analysis of the manifestations of the polymorphic marker rs659366 UCP2 gene in patients with type 2 diabetes and obesity showed that the genotype of T/T and C/T is characterized by a low content of vitamin C in the serum than genotype of C/C. Polymorphism rs1801133 of MTHFR gene in patients with type 2 diabetes and obesity revealed that genotype C/T and T/T showed a lower content of vitamins B6, B12, folate in serum as compared with those indices in genotype C/C.Conclusion. The recommendation of vitamin-mineral complexes to patients with type 2 diabetes and obesity should be quided by molecular genetic studies, which might individually appoint the necessary micronutrients to improve the metabolic performance and reduce the risk of vascular complications in these patients.