Abstract
INTRODUCTION: A number of biochemical and medical researchers have detected increased activity of tyrosinase in skin tumor cells. The most famous and available inhibitor, kojic acid, has several side effects and is not completely safe for use.OBJECTIVES: This paper describes the study of inhibitory influence of halogen boroxine K2[B303F4OH] on tyrosinase. The research was prompted by the ability of this compound to inhibit enzymes through metal ion chelation as well as its synthesis and application in cosmetic skin products that produce no serious side-effects.METHOD: Tyrosinase activity was measured by spectrophotometric analysis for the appearance of dopachrome pigment at a wavelength of 475 nm. Tyrosinase exhibited typical Michaelis-Menten kinetics.RESULTS: Tests of the proposed inhibition of the enzyme tyrosinase showed that K2[B3O3F4OH] had weak inhibitory properties.CONCLUSION: It will be necessary to search for new ways of antitumor mechanisms that differ from those of previous results.
Highlights
A number of biochemical and medical researchers have detected increased activity of tyrosinase in skin tumor cells
It will be necessary to search for new ways of antitumor mechanisms that differ from those of previous results
Tyrosinase was named because of the activity of the amino acid, tyrosine, which is found in most animal cells and is very important in melanin synthesis (Uchidaa, Ishikawa, & Tomoda, 2014)
Summary
A number of biochemical and medical researchers have detected increased activity of tyrosinase in skin tumor cells. According the World Health Organization, two to three million people are diagnosed with some form of malignant skin tumor each year, with 41,000 patients dying each year from melanoma. Numerous people suffer from many form of skin disorders (www.skincancer.com). Most investigated is the enzyme, tyrosinase (EC number 1.14.18.1), which is thought to play a main role in skin disorders (Saran et al, 2004). Tyrosinase was named because of the activity of the amino acid, tyrosine, which is found in most animal cells and is very important in melanin synthesis (Uchidaa, Ishikawa, & Tomoda, 2014). Human tyrosinase is a single membrane-spanning transmembrane protein, and when tyrosine forms the substrate, it forms dopaquinone, an intermediate in the production of the pigment, melanin. Dopaquinone spontaneously forms an orange-red pigment called dopachrome, which undergoes a final reaction to form the blackish brown pigment, melanin
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