Abstract

AbstractThe high intake of antitubercular isonicotinyl hydrazide (INH) is hazardous to human body. Hence, there is a need for its detection. For this reason, we study interaction between human hemoglobin (HHb) and INH by applying fluorescence and UV–vis spectroscopic techniques. Fluorimetric as well as UV–vis results showed the formation of complex between HHb and INH. The proposed HHb‐based fluorescence method was applied directly for the determination of INH in pharmaceutical sample.

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