Study of inhibitory effect of Pien Tze Huang on human colon cancer SW480 cell line

Abstract

Objective To explore the Pien Tze Huang(PTH) inhibitive effect of human colon cancer SW480 cell line. Methods Throug the human colon cancer SW480 cell line in vitro cultivation, we randomly set five group, which was blank group, 5-FU group and others in the differernt doses of PTH, respectively adopt the corresponding drug intervention for 24 h, 48 h, 72 h, then use the cell proliferation assay (MTT method) and fluorescence microscope to observe the inhibition effect. The animal model was established by using SW480 human colon cancer cells, and we randomly divided 150 mice into model group, 5-FU group and PTH low, medium and high dose group, each group of thirty. To explain the inhibitory effect by anti-tumor rate and in situ apoptosis (TUNEL method) .We use three days of external drug intervention. Results The inhibitory rates of 1 mg/mL, 2 mg/mL and 3 mg/mL PTH on human colon cancer SW480 cells in vitro were 70.05%, 71.39% and 70.12%, which were significantly different from those in 5-FU group (χ2=4.49, 4.97, 4.52; all P 0.05). Fluorescence microscopy showed that the number of SW480 cell lines in 5-FU group and 5-FU group decreased in varying degrees, with smaller volume, poor refractive index, cell suspension and exfoliation death. The inhibition rates of high, medium and low dose groups of PTH on SW480 cells were 51%, 39% and 23% respectively. There was no significant difference between high and medium dose groups and 5-FU group (χ2=0.05, 0.49; all P>0.05), but there was significant difference between low dose group and low dose group (χ2=4.09, P<0.05). Each dose group and group 5-FU had different degrees of apoptosis on human colon cancer SW480 cells. Conclusion PTH have significant inhibitive effect on human colon cancer SW480 cell in vivo, and the inhibitory effect of high and middle doses is similar to that of 5-FU, which is better than low dose. Therefore, the effect of high dose could be considered better, and the inhibitory effect in vitro is better than that of 5-FU, but the effect of dose difference is not significant. Key words: Colonic neoplasms; Antineoplastic agents; Pien tze huang; SW480 cell line; Antitumor effect