Study of influence of interferon-γ combined with lauromacrogol on inhibiting proliferation and inducing apoptosis of endothelial cells

Abstract

Objective To investigate the effects and mechanism of interferon-γ (IFN-γ) and lauromacrogol on proliferation, apoptosis, invasion and migration of endothelial cells. Methods Different concent rations of IFN-γ and lauromacrogol were added to intervene endothelial cells for 48 h. The proliferation and apoptosis were detected by methyl thiazolyldiphenyl-tetrazolium bromide (MTT) assay and flow cytometry. The migration and invasion of endothelial cells were observed by using scratch test and Transwell assay. Western blot was used to detece the expression of B cell lymphoma/leukemia-2 associated X protein (bax), B cell lymphoma/leukemia-2 (bcl-2), and cysteine-containing aspartate-specific proteases (Cleaved Caspase-9) protein. Results The proliferation of endothelial cells in hemangioma was significantly inhibited by different concentrations of lauromacrogol and IFN-γ. Compared with 0 μg/ml or 0 μg/L (0.836±0.088; 0.809±0.062), the difference was statistically significant (tLauromacrogol 20 μg/ml=2.952, PLauromacrogol 20 μg/ml=0.042; tLauromacrogol 30 μg/ml=4.588, PLauromacrogol 30 μg/ml=0.010; tLauromacrogol 40 μg/ml=7.351, PLauromacrogol 40 μg/ml=0.002; tLauromacrogol 50 μg/ml=12.939, PLauromacrogol 50 μg/ml=0.000; tIFN-γ 20 μg/L=3.720, PIFN-γ 20 μg/L=0.021; tIFN-γ 40 μg/L=4.684, PIFN-γ 40 μg/L=0.009; tIFN-γ 60 μg/L=9.539, PIFN-γ 60 μg/L=0.001; tIFN-γ 80 μg/L=14.039, PIFN-γ 80 μg/L=0.000). Lauromacrogol significantly inhibit the proliferation of endothelial cells with optimum concentration were 30 μg/ml and 40 μg/L. The effect of combination on reducing apoptosis, decreasing the ability of invasion and migration, reducing bcl-2 protein, and increasing bax, Cleaved Caspase-9 proteins were more pronounced (P凋亡=0.000, P迁移=0.000, P侵袭=0.000). Conclusion IFN-γ and lauromacrogol can inhibit proliferation and induce apoptosis of endothelial cells, reduce the invasion and migration, which may be an approval to decrease expression of bcl-2, and increased bax, Cleaved Caspase-9. Key words: Interferon-γ; Lauromacrogol; Endothelial cells; B cell lymphoma/lewkmia-2