Abstract
Objective: The systematic study of effective alternative anti-diabetic drugs has great importance to manage diabetes as well as other oxidative stress-related diseases. According to previous research, root and bark of Mussaenda macrophylla plant has anti-microbial, anti-coagulant, anti-inflammatory, and hepatoprotective activity. Ethnomedicinal data shows that Mussaenda macrophylla is used to treat diabetes as well as oxidative stress. The objective of this research is to investigate in vitro anti-diabetic and anti-oxidant activity of root extract of Mussaenda macrophylla.
 Methods: DPPH free radical scavenging assay was used to detect anti-oxidant potency of ethanol and methanol root extract of the plant and expressed as % of radicle inhibition. Anti-diabetic activity was determined by the glucose diffusion method using a glucose oxidase kit and results were expressed as mean±SD.
 Results: The ethanol root extract at the concentration of 50 mg/ml and 100 mg/ml showed better glucose diffusion inhibition than that of methanol extract at the same concentration on increasing time interval. Ethanol extract at the concentration 100 µg/ml displayed better DPPH scavenging activity (89.83±0.19 %) than that of methanol extract (86.61±0.75%).
 Conclusion: This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model.
Highlights
Oxidative metabolism is essential for the survival of cells and plays a key role in the normal in vivo regulatory system
This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model
Ethanol extract of root was found to be more effective to inhibit DPPH free radical compared to methanol extract in all tested concentrations and its antioxidant potency is almost similar to ascorbic acid, which is supported by fig
Summary
Oxidative metabolism is essential for the survival of cells and plays a key role in the normal in vivo regulatory system. Accumulation of excess free radicals may result in excess production of some enzymes like superoxide dismutase, catalase, and peroxidase, leading apoptosis by oxidation of membrane lipids, cellular proteins, DNA, and enzymes, shutting down cellular respiration. They can alter cell signaling pathways [1]. Antioxidant has the capability to trap free radicals Secondary metabolites such as flavanoids and flavones are broadly distributed in plants and have anti-oxidant properties [4]. May contain natural antioxidant constituents [4]. The genus Mussaenda has been a significant source of natural products in the field of pharmacology [6]
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