Study of immunogenic properties of experimental series of bivalent vaccine against Carnivorous leptospirosis in laboratory conditions

Abstract

Leptospirosis is an infectious natural-focal disease of many species of animals and human with a wide range of clinical signs. The most common serovars causing leptospirosis in dogs are Icterohaemorrhagiae and Canicola. Analyzing the epizootic situation concerning leptospirosis of carnivores in Ukraine, employees of the Laboratory of Leptospirosis of Institute of Veterinary Medicine of the National Academy of Agrarian Sciences have developed and produced successively three series of bivalent inactivated vaccine against leptospirosis of carnivores, taking into account the etiological structure of the disease of this animal species. The paper presents the results of the study of three experimental series of inactivated polyvalent vaccine against leptospirosis of carnivores regarding: pH, sterility, residual amounts of inactivant, completeness of inactivation, harmlessness and immunogenic activity. It has been established that according to the indicators of sterility, completeness of inactivation, residual amount of inactivant, harmlessness and concentration of hydrogen ions, all three series of vaccine meet the requirements and norms of normative documentation. Intravenous administration of all three experimental series of the vaccine to the experimental rabbits provided the formation of specific anti-leptospirosis antibodies in the titers corresponding to the parameters of the immunogenicity norms established in the technical conditions of the drug.