Study of immunogenic properties of B. suis 245 vaccine strain on domestic reindeer

Abstract

This article presents the results of the study of immunogenic properties of the museum vaccine strain Brucella suis 245, from the collection of museum strains of brucella at All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko, which is of particular interest in anti-epizootic efficacy in the system of prevention and control of brucellosis of reindeer, as it is a homologous strain. Currently, a vaccine from the heterogeneous strain B. abortus 82 is used to prevent reindeer brucellosis. The subcutaneous injection of the Brucella suis 245 vaccine causes a local and general reaction of the body, as in the case of heterogeneous strains, the use of the vaccine at the root of the tongue (per os) eliminates all side effects. In the first days after the injection, the culture of the vaccine strain B. suis 245 is well settled in the reindeer. The humoral immune response of the body of the reindeer was studied within 15, 30, 75, 310 days after immunization by testing animal blood serum on brucellosis in RBT, SAT, Complement fixation test and immunodiffusion test with O-PS antigen. Agglutinating antibodies, detected in SAT, were detected in all animals on the 15th day after immunization and their levels in groups were as following: 1gr. - 466.6; 2 gr. - 150.0; 3 gr. - 125.0; 4 gr. - 100.0; 5 gr. - 233.3; 6 gr. - 100.0 ME. A decrease in SAT below the diagnostic level was observed in oral vaccinated animals after 75 days; and in animals vaccinated subcutaneously at 10 billion. m.k. with vaccine from strain 19 - in 310 days. In the body of reindeer grafted under the subcutaneous method, complementary binding antibodies appear on the 15th day after the injection of the vaccine, reaching the maximum level by 30 and the minimum level by 310 days.