Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse.

Abstract

Immunoglobulin E (IgE) is the least abundant antibody isotype in mammalians, yet it plays a critical role in allergy and asthma. IgE-producing (IgE+) B cells are rare and difficult to detect, which have hindered progress to understand their generation and differentiation. Recently developed new fluorescent IgE reporter mice have enabled better understanding of the biology of IgE+ B cells. We here describe the usage of the Verigem IgE reporter mouse to study IgE+ B cells and plasma cells by flow cytometry and microscopy.