Study of IDH1 (R132H) Mutation and Expression of ATRX Marker in Correlation with the Histopathological Grading in Gliomas

Abstract

Background: The third highest cancer associated mortality and morbidity rates are associated with primary malignant brain tumors among lives worldwide. Newer WHO CNS tumor classification shows that mutation of IDH1 is also a predictive factor. The presence of IDH1 alteration results in an increased progression free survival in gliomas. Similarly, ATRX mutations are associated with favorable outcome in astrocytic tumors. The present study makes an attempt to assess the correlation between IDH1 mutation and ATRX expression and histopathological grading in glial tumors which helps us to grade glial tumors in comparison with present day molecular advancements in a better way.Objectives: To develop an understanding of the relationship between IDH1 and ATRX expression with the histopathological grades of glial tumors on immunohistochemistry.Methods: The present study is an observational, analytical and retrospective study to be conducted for a duration of two years, in the Histopathology and Immunohistochemistry division of the Pathology Department, Jawaharlal Nehru Medical College, Sawangi (Meghe), in coordination with the Department of Neurosurgery, Acharya Vinoba Bhave Rural Hospital, Sawangi (Meghe). In this study, approximately 45-50 resected specimens from suspected cases of Glial tumours received in the Department of General Pathology, J.N.M.C. will be taken. We will evaluate the correlation between IDH1 mutation and ATRX expression with the histopathological grades in glial tumors in comparison with the present-day molecular advances on immunohistochemistry for better understanding through a well-tabulated master chart.Results: The observations will be depicted in a well-tabulated master chart.Conclusion: Conclusion will be elicited from the results procured from the research.