Abstract
Diamond-like carbon (DLC) coatings are extremely useful for creating biocompatible surfaces on medical implants. DLC and silicon doped DLC synthesised on silicon wafer substrate by using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of HSA with doped and undoped DLC films have been investigated. The chemical composition of the surface before and after adsorption was analysed using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR). Results showed that silicon incorporation DLC tends to increase of sp3/sp2 hybridization ratio by decreasing sp2 hybridized carbon bonding configurations. Following exposure to solutions containing (0.250 μg/ml) HSA, the results indicated that significant changes in the C, N and O levels on the surfaces with reducing of the Si2p band at 100 eV. From FTIR spectrum, the peaks occur the following functional groups were assigned as amide I and II groups at 1650 cm-1 and 1580 cm-1. Both XPS and FTIR spectroscopy confirm that HSA was bound onto the surfaces of the DLC and Si-DLC films via interaction of ionized carboxyl groups and the amino group did not play a significant role in the adsorption of protein. These results from peak intensity show that an adsorbed layer of HSA is higher at high level (19%) silicon doping. Therefore doping of DLC may provide an approach to controlling the protein adsorption.
Highlights
Analysing the interaction of proteins with the surfaces of materials intended for biomedical applications is fundamental for understanding cellular events and the overall host response
From Fourier transform infrared (FTIR) spectrum, the peaks occur the following functional groups were assigned as amide I and II groups at 1650 cm−1 and 1580 cm−1. Both X-ray photoelectron spectroscopy (XPS) and FTIR spectroscopy confirm that Human serum albumin (HSA) was bound onto the surfaces of the Diamond-like carbon (DLC) and Si-DLC films via interaction of ionized carboxyl groups and the amino group did not play a significant role in the adsorption of protein
We explore the coating of silicon wafer substrate with DLC and silicon doped DLC thin films using plasma enhanced chemical vapour deposition (PECVD)
Summary
Analysing the interaction of proteins with the surfaces of materials intended for biomedical applications is fundamental for understanding cellular events and the overall host response. The importance of a variety of molecules in the biomedical field is known for several applications, including drug delivery, biomaterials, extracorporeal therapy and solid-phase diagnostics [1]. Both the nature of the biomaterial and the protein has effects on the adsorption process. The most significant surface properties of biomaterials, including the elemental composition, functional groups and surface energy are very important. All of these parameters play a role in enhancing or decreasing the adsorption of proteins or biological compounds during the implant. DLC possess a unique combination of desirable properties including chemical inertness, high density, heamocompatibility and poor coefficient of friction [7]
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