STUDY OF HEAVY METAL AND HETEROCYCLIC COMPOUND FISH IN AROMATIC MILKFISH AMINE (CHANOS CHANOS) FRIED IN USED COOKING OIL

Abstract

Although the heterocyclic aromatic amines in fish processing has been widely reported, yet these compounds in milkfish fried with used cooking oil is still lacking. Therefore, this study is aimed to analyze the content of heterocyclic aromatic amine compounds along with the heavy metals (Ni, Pb, Hg and Cd) in milkfish fry kantinogen treated with cooking oil. Chemical components were identified according to Gross method using gravity chromatography with gel 60 as the stationary phase and purified by column Sephadex LH20, and checked by UV spectrometry, IR, and HPLC. The compound PhIP (2-Amino-I-Methyl-6-Fenilnidazo [4,5-b] pyridine), obtained by analysis, is thought to be caused by the influence of cooking oil. By atomic absorption spectrophotometer was nickel (Ni) with an average concentration of 1.6075 ± 0.2405 mg/kg. The role of nickel compounds in milkfish fry genotoksit is suspected as a trigger effect on PhIP compound. Keyword : Fried Milk Fish, Reused Oil, Nickel, PhIP.