Study of heart rate and corticosterone variability in the simulation of rhino-surgical interventions in biological objects.

Abstract

Aims: to assess changes in the time range of heart rate variability (HRV) and plasma corticosterone concentration in rats after simulating septoplasty. Materials and methods: a septoplasty was simulated in 30 mature male Wistar rats weighing 210-290 g. An ECG was recorded with subsequent analysis of the time domain of HRV, as well as blood sampling for changes in the concentration of corticosterone in the blood plasma. Results: SDNN significantly increased in comparison with the control on days 2 and 3 (p <0.001), but decreased on days 4-5 (p <0.001) and 6 days (p <0.01 ). rMSSD changed in waves with two irregular peaks on days 1 and 6. SDNN / rMSSD, in comparison with the 1st day of the postoperative period, increased on the 2nd day and continued to grow (p <0.05), and on the 4th day it began to decrease (p <0.01). The total power of HRV was low throughout the postoperative period (p <0.001), except for the 3rd day, when it was equal to the control data. The increase in the total power indicator fell on the 3rd day after the operation (p <0.01), after which its decline occurred again. The concentration of corticosterone in the blood plasma in rats was significantly higher than before (p <0.001). From the 2nd to the 4th postoperative day, its plateau was determined. Conclusion: Simulation of septoplasty leads to changes in the time range of HRV, an increase in the concentration of corticosterone in the blood plasma in rats with its maximum at the time of surgery and after 24 hours, the formation of a "plateau" from the 2nd to the 4th postoperative days, which coincides with changes of HRV.