Study of haematology profile & histopathological changes in di-ammonium phosphate induced climbing perch, Anabas testudineus (Bloch.)

Abstract

The current study includes the histopathological and haematopathological alterations induced by chronic (20 days) exposure of the fish Anabas testudineus to a sublethal concentrations (0.092 gm/L) of inorganic fertilizer, Di-ammonium phosphate (DAP). Behaviour response showed in test fishes like lost their natural colouration, loss of equilibrium, jerk movement before death. At haematological levels various parameters were analysed such as Hb, RBC, WBC decreases while in DLC, Neutrophil, Monocytes and Eosinophil values increase. For the histopathological study some vital organs were taken i.e. kidney, liver, intestine, testis and ovary. The present study showed major significant histopathological alteration like vacuolar degenerative changes, necrosis etc. found in liver, intestine, testis, ovary and kidney organs dysfunction in response to DAP toxicity effect in the fish A. testudineus. So, it is suggested that more suitable to culture at water fertilizer, Di-ammonium phosphate concentration of < 0.092 g/l for optimum growth performance. The information will be major role on different levels of responses of organisms with respect to pollutant stress is a necessary pre-requisite for the proper management of fertilizer application in agriculture and aquaculture.