Abstract
The yeastPhaffia rhodozyma, known for its ability to synthesize carotenoids, was adapted and cultivated in liquid-phase media using peat hydrolysates as the main substrate source. The hydrolysates were prepared using high-pressure treatment at 185°C, without the addition of acid. The growth of the yeast was studied as a function of the pH, temperature, culture time, and agitation speed. The best conditions for the growth of the yeast were: a pH of 7, a temperature of 18°C, 5-d culture time, and 200-rpm agitation. Under those conditions, P.rhodozyma produced a concentration of 1279.82 μg carotenoids g-1 dry yeast, which compares well with other previously reported results.
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