Study of growth, invasion and metastasis on ovarian epithelial cancer cell line with CCL18 over-expression by mediated in vitro

Abstract

To investigate the effect of growth, invasion and metastasis on ovarian cancer cell line SKOV3 with chemokine (C-C motif) ligand 18 (CCL18) over-expression by mediated in vitro. The restructuring plasmid of CCL18 expression was constructed and SKOV3 cells was transfected with plasmid DNA in vitro. The growth curve, cell cycle, cell migration, invasion and adhesion capacity in SKOV3-CCL18 cells and control cells were detected by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, transwell chamber, migration invasion and fibronectin adhesion method, respectively. (1) CCL18-pEGFP-N1 plasmid was successfully constructed and transmitted to the SKOV3 cells, the stable growth of the subculture SKOV3-CCL18 cells was screening and completed. (2) Compared with SKOV3-CCL18 cells and SKOV3 or SKOV3-pEGFP-N1 cells, there was not differences statistically significant in growth curve (all P > 0.05); but the rate of the SKOV3-CCL18 cells in a proliferative field (S+G2+M) was significantly higher than that in SKOV3 or SKOV3-pEGFP-N1 cells (32.80% versus 27.06%, 32.80% versus 26.98%, respectively; all P < 0.05). (3) The invasion, migration and adhesion capacity of SKOV3-CCL18 cells (being 0.49 ± 0.18, 1.16 ± 0.25 and 0.39 ± 0.10, respectively) in vitro were significantly higher than those in SKOV3 (being 0.23 ± 0.13, 0.36 ± 0.10 and 0.16 ± 0.03, respectively) or SKOV3-pEGFP-N1 cells (being 0.19 ± 0.05, 0.38 ± 0.23 and 0.13 ± 0.11, respectively; all P < 0.05). The CCL18 over-expression in epithelial ovarian cancer SKOV3 cells could lead to strengthen ability of invasion, migration and adhesion in vitro.