Study of Gibberellic Acid Production by Solid State Fermentation Using Fusarium Moniliforme Sheldon

Piyushbhai Vishnubhai Desai,Rakeshkumar Ramanlal Panchal

doi:10.3126/ijasbt.v4i3.15588

Abstract

Gibberellic acid production using Fusarium moniliforme, isolated from wilted sugarcane plant has been investigated by solid state fermentation (SSF). The gibberellic acid production of 154mgm/gm was obtained on commercial wheat bran (CWB) mineral salt acid bed in 500 ml flasks after 168 h incubation. The gibberellic acid production rate was about 0.6 to 0.9 mgm/gm/hr during 96 to 168 h. Different carbon sources namely sucrose, lactose, maltose, soluble starch, glycerol, wheat flour and maize flour were tested as an additional substrate along with CWB at the concentration of 25% w/w or v/w base to observe its effects on gibberellic acid production. Soluble starch has been proved the best additional carbon source for gibberellic acid production, which yielded 1160mgm/gm of gibberellic acid after 168 h. Similarly, various nitrogen sources namely NH4Cl, NH4NO3, (NH4)2SO4, (NH4)MoO4 and urea were tested as an additional substrate at the concentration of 0.07% w/w of CWB. Urea was proved as the best nitrogen source which yielded 532 mgm/gm of gibberellic acid after 168 h incubation. We have observed about 7.5-fold and 3.5-fold increase in gibberellic acid production upon addition of soluble starch and urea respectively, in CWB using Fusarium moniliforme.Int J Appl Sci Biotechnol, Vol 4(3): 402-407

Highlights

A group of diterpenoid acids termed as Gibberellic acids (GAs) are largely produced by Fusarium moniliforme which is earlier known as Gibberella fujicuroi, and functions as growth regulators of plants
The origin of research in to gibberellins can be traced to Japanese plant pathologists who were investigating the causes of the ‘bakanae’ disease which seriously lowered the yield of rice crops in Japan, Taiwan and throughout the Asian continent (Kurosawa, 1926)
There was about a 7 to 8 fold increase in the GA3 production when commercial wheat bran (CWB) was enriched with soluble starch

Summary

Introduction

A group of diterpenoid acids termed as Gibberellic acids (GAs) are largely produced by Fusarium moniliforme which is earlier known as Gibberella fujicuroi, and functions as growth regulators of plants. The first paper on the cause of bakanae was published in 1898 by Shotaro Hori who demonstrated that the symptoms were induced by infection with a fungus belonging to the genes Fusarium, probably Fusarium heterosporium Nees. Elichi Kurosawa (1926) found that culture filtrates from dried rice seedlings caused marked elongation in rice and other sub-tropical grasses. He concluded that bakanae fungus secretes a chemical that stimulates shoot elongation, inhibits chlorophyll formation and suppresses root growth

Objectives

Methods

Results