Abstract
This study was undertaken to confirm whether early pregnancy factor (EPF)-like substance(s) was secreted by fertilized bovine ova. Using the rosette inhibition test, EPF-like substance(s) was detected in the culture media of fertilized bovine ova, while this substance was not found in the culture media of unfertilized bovine ova. EPF-like activity, analyzed by HPLC-gel permeation chromatography, was present between molecular weights of 16 and 37 KD. In conclu-sion, EPF-like substance(s) is secreted by fertilized bovine ovum and has a molecular weight of16-37 KD.
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